Assist. Prof David Kinahan

Biography

David Kinahan is an Assistant Professor in the School of Mechanical and Manufacturing Engineering since October 2018. David completed a BEng in Aeronautical Engineering (2003) and a PhD (2008) at University of Limerick (UL). His PhD focused on high-throughput droplet microfluidics for DNA analysis.

In late 2007 David joined Stokes Bio Ltd, a spin-out from UL, as a Senior Engineer, and was later promoted to Engineering Manager leading a team of 10 engineers.

In January 2012 David joined the Biomedical Diagnostics Institute  in DCU and since then has applied microfluidics to a wide range of application areas including human health (HIV diagnostics, CTC detection, CVD diagnostics, liver disease, early detection of bacterial meningitis) and point-of-use testing (plant pathogen detection, environmental monitoring etc).

From January 2017 until October 2018 David was a Group Leader within Fraunhofer Project Center at Dublin City University ([email protected]).

Research Expertise

PhD Students

Select Publications

Event-triggered logical flow control for comprehensive process integration of multi-step assays on centrifugal microfluidic platforms
  Kinahan, D. J., Kearney, S. M., Dimov, N., Glynn, M. T., & Ducrée, J.      2014      Lab on a Chip

The centrifugal “lab-on-a-disc” concept has proven to have great potential for process integration of bioanalytical assays, in particular where ease-of-use, ruggedness, portability, fast turn-around time and cost efficiency are of paramount importance. Yet, as all liquids residing on the disc are exposed to the same centrifugal field, an inherent challenge of these systems remains the automation of multi-step, multi-liquid sample processing and subsequent detection. In order to orchestrate the underlying bioanalytical protocols, an ample palette of rotationally and externally actuated valving schemes has been developed. While excelling with the level of flow control, externally actuated valves require interaction with peripheral instrumentation, thus compromising the conceptual simplicity of the centrifugal platform. In turn, for rotationally controlled schemes, such as common capillary burst valves, typical manufacturing …

 

Paper imbibition for timing of multi-step liquid handling protocols on event-triggered centrifugal microfluidic lab-on-a-disc platforms
  Kinahan, D. J., Kearney, S. M., Faneuil, O. P., Glynn, M. T., Dimov, N., & Ducrée, J.      2015      RSC Advances

Rotational microfluidic platforms have attracted swiftly growing interest over the last decade due to their suitability for integration and automation of sample preparation and detection. Valving is of pivotal importance on these compact “Lab-on-a-Disc” (LoaD) platforms as all liquids are exposed to the same centrifugal field. A number of valving technologies have been developed to coordinate timing of serial and/or parallel multi-step/multi-liquid assay protocols comprising of laboratory unit operations (LUOs) such as the release, metering and mixing of sample and reagents. So far these valving techniques could be broadly categorised into rotationally controlled or externally actuated schemes. Only recently a new, “event-triggered” flow control has been introduced. In this approach, a valve is opened upon arrival of a liquid at a defined destination on the disc; this innovative mechanism for the first time permits the …

 

Density-gradient mediated band extraction of leukocytes from whole blood using centrifugo-pneumatic siphon valving on centrifugal microfluidic discs
  Kinahan, D. J., Kearney, S. M., Kilcawley, N. A., Early, P. L., Glynn, M. T., & Ducree, J.      2016      PloS one

Here we present retrieval of Peripheral Blood Mononuclear Cells by density-gradient medium based centrifugation for subsequent analysis of the leukocytes on an integrated microfluidic “Lab-on-a-Disc” cartridge. Isolation of white blood cells constitutes a critical sample preparation step for many bioassays. Centrifugo-pneumatic siphon valves are particularly suited for blood processing as they function without need of surface treatment and are ‘low-pass’, ie, holding at high centrifugation speeds and opening upon reduction of the spin rate. Both ‘hydrostatically’and ‘hydrodynamically’triggered centrifugo-pneumatic siphon valving schemes are presented. Firstly, the geometry of the pneumatic chamber of hydrostatically primed centrifugo-pneumatic siphon valves is optimised to enable smooth and uniform layering of blood on top of the density-gradient medium; this feature proves to be key for efficient Peripheral …

 

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